ACSS1 Knockout cell line (Hep G2)

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ACSS1 Knockout cell line (Hep G2) Order

ACSS1 Knockout cell line (Hep G2)
Catalog#
YKO-HT5365
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
ACSS1 Knockout cell line (Hep G2)
Order

Detailed Product Information

Product category
KO Cell Lines
Cell name
ACSS1 Knockout cell line (Hep G2)
Species
Human
Gene Name
ACSS1
Gene ID
84532
Gene Information
NCBI
Construction method
Electroporation/Lentivirus
Mycoplasma test
negative
Target region
Knockout region: Exon 1 (based on transcript ACSS1-201)
Validation: PCR+Sanger Sequencing

Instruction

Use exclusive Red Cotton system to design a sgRNA fragment knockout strategy for the ACSS1 gene, clone the two sgRNAs into CRISPR-U™ vector, transfect the vector into the Hep G2 cell line by electroporation/lentivirus method, use the limited dilution method for single-cell cloning and use the EZ-editor™ Monoclone Genotype Validation Kit (Cat# YK-MV-1000) for nucleic acid lysis and PCR amplification on the single-cell clones, followed by Sanger sequencing to verify the genotype. Upon passing the verification, ACSS1 gene knockout ACSS1 Knockout cell line (Hep G2) will be expanded and cryopreserved.
Knockout Cell Line Work Flow and Validatio Knockout Cell Line Work Flow and Validatio
ACSS1 Knockout cell line (Hep G2)
ACSS1 Knockout cell line (Hep G2)
Catalog#
YKO-HT5365
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
Order

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  • ACSS1 Knockout cell line (HEK293)
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    Organism:
    Gene Name: ACSS1
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  • ACSS1 Knockout cell line (HEK293)
    Request a Quote More details

    Organism: Human
    Gene Name: ACSS1
    Gene ID: 84532
    Size: 1*10^6
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